Determination of Usinic Acid by HPLC

Xi’an QingShuo Import And Export Trade Co.,Ltd.

• HPLC Conditions:

Column: Inertsil ODS-3 4.6×150mm 5μm

Flow rate: 1.0mL/min

Detection: 284nm

Mobile phase: Methanol: 0.1% phosphoric acid-water solution (91: 9, V/V )

Injection Volume: 10μl

• Solution Preparation

1. Standard Preparation:

Accurately weigh approximately 10mg of Usinic Acid Standard into a 50ml volumetric flask. Dissolve and Dilute to volume with chloroform.

2. Sample Preparation:

Accurately weigh approximately 10mg of Usinic Acid into a 50ml volumetric flask. Dissolve and Dilute to volume with chloroform. Filter through a 0.45μm filter into an HPLC vial or centrifuge to obtain a clear test solution.

III. Calculation

The final results are quantified against external standard.